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Separation and Quantitation of R and SEnantiomers using Normal PhaseChromatography on an API 3000™LC/MS/MS System Normal phase chromatography can
strates that it is possible to safely and be used for the analysis of chiral
compounds using the API 3000™
LC/MS/MS System.
Key Features
Figure 1. Verapamil calibration curves for enantiomer R and S.
Figure 2. Methylphenidate calibration curves for enantiomer R and S.
Experimental Conditions
APCI (Nitrogen gas used for all connections) animals dosed with racemic mixtures.
the coronary vasodilator, verapamil.
Figure 3. Fluoxetine calibration curves for enantiomer R and S.
Figure 4. Verapamil, 0.1 ng/mL racemate concentration, extracted from
Figure 5. Methylphenidate, 0.1 ng/mL racemate concentration,
rat plasma.
extracted from rat plasma.
Results and Discussion
centration range of 0–1,000 ng/mL.
separation, approximately 65 minutes.
Worldwide Sales Offices
Applied Biosystems vast distribution
office locations, please call the division at
Figure 6. Fluoxetine, 100 ng/mL racemate concentration, extracted from rat
plasma and fluoxetine, 10 ng/mL racemate concentration, extracted from rat
plasma plus integrating a switching valve to overcome mobile phase incom-

patibility. Note that the intensity is comparable despite the ten-fold
decrease in analyte concentration.
Not for use in diagnostic procedures.
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Prozac is a registered trademark of Eli Lilly and company.
Ciba-Geigy Corporation.
Daicel is a trademark and Chiralpak is a registered trademark of Daicel ChemicalIndustries, Ltd.
Sigma is a registered trademark of Sigma Chemical Company.
2000 Applied Biosystems. All rights reserved. Information subject to change without notice. Printed in the USA, 12/2000, BHLPublication 114AP12-01


Mdr1 gene c3435t polymorphism and the risk of acquired aplastic anaemia

British Journal of Haematology, 2002, 117, 768–774PROLONGED COMPLETE REMISSION IN TWO CASES OF ACUTE PROMYELOCYTIC LEUKAEMIATREATED WITH ATRA ALONEThe use of all-trans retinoic acid (ATRA) therapy forplatelet count was normal. On day 58, bone marrowinduction for acute promyelocytic leukaemia (APL) is wellshowed morphological remission, with normal cytogeneticsestablished and leads to a com


ß-Glucuronidase Immunotherapy in Dust Mite Allergic Children A Double-Blind Randomized Placebo- Controlled Trial With Short-Term ß-Glucuronidase Therapy in Children With Chronic Rhinoconjunctivitis and/or Asthma Due to Dust Mite E Galli,1 MS Bassi,2 E Mora,3 M Martelli,4 S Gianni,1 G Auricchio,1 1 Research Center San Pietro, Fatebenefratelli Hospital, AfaR, Rome, Italy4Santa

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