L’ivermectine (Stromectol) est un antiparasitaire dont l’action repose sur la liaison sélective aux canaux chlore activés par le glutamate présents dans les cellules nerveuses et musculaires des parasites. Cette fixation entraîne une augmentation du flux de chlore, provoquant une hyperpolarisation et une paralysie irréversible. L’ivermectine est active contre la gale, l’onchocercose et certaines strongyloïdoses. Sa biodisponibilité orale est variable, augmentée par la prise alimentaire, et son élimination est principalement fécale via un métabolisme hépatique. Elle ne traverse pas la barrière hémato-encéphalique, ce qui limite les effets neurologiques chez l’homme. Les précautions concernent l’interaction avec les inhibiteurs du CYP3A4, ainsi que les réactions inflammatoires dues à la destruction massive des parasites. Dans les documents de référence, stromectol prix est associé à des protocoles précis adaptés aux différentes infestations, avec une attention particulière sur la sécurité d’emploi en cas d’immunodépression.

114ap12.qxd

APPLICATION NOTE
Separation and Quantitation of R and SEnantiomers using Normal PhaseChromatography on an API 3000™LC/MS/MS System Normal phase chromatography can
strates that it is possible to safely and be used for the analysis of chiral
compounds using the API 3000™
LC/MS/MS System.
Overview
Key Features
Figure 1. Verapamil calibration curves for enantiomer R and S.
APPLICATION NOTE
Figure 2. Methylphenidate calibration curves for enantiomer R and S.
Experimental Conditions
Experiment
APCI (Nitrogen gas used for all connections) animals dosed with racemic mixtures.
Verapamil
Methylphenidate
Fluoxetine
the coronary vasodilator, verapamil.
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Figure 3. Fluoxetine calibration curves for enantiomer R and S.
Figure 4. Verapamil, 0.1 ng/mL racemate concentration, extracted from
Figure 5. Methylphenidate, 0.1 ng/mL racemate concentration,
rat plasma.
extracted from rat plasma.
Results and Discussion
centration range of 0-1,000 ng/mL.
separation, approximately 65 minutes.
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office locations, please call the division at www.appliedbiosystems.com.
Figure 6. Fluoxetine, 100 ng/mL racemate concentration, extracted from rat
plasma and fluoxetine, 10 ng/mL racemate concentration, extracted from rat
plasma plus integrating a switching valve to overcome mobile phase incom-
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patibility. Note that the intensity is comparable despite the ten-fold
decrease in analyte concentration.
Conclusions
Not for use in diagnostic procedures.
Applied Biosystems is a registered trademarkof Applera Corporation or its subsidiaries in the US and certain other countries. MDS and SCIEX are trademarks of MDS Inc.
Prozac is a registered trademark of Eli Lilly and company.
Ciba-Geigy Corporation.
Daicel is a trademark and Chiralpak is a registered trademark of Daicel ChemicalIndustries, Ltd.
Sigma is a registered trademark of Sigma Chemical Company.
2000 Applied Biosystems. All rights reserved. Information subject to change without notice. Printed in the USA, 12/2000, BHLPublication 114AP12-01 www.appliedbiosystems.com

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