APPLICATION NOTE
Separation and Quantitation of R and SEnantiomers using Normal PhaseChromatography on an API 3000™LC/MS/MS System
Normal phase chromatography can
strates that it is possible to safely and
be used for the analysis of chiral compounds using the API 3000™ LC/MS/MS System. Overview Key Features Figure 1. Verapamil calibration curves for enantiomer R and S. APPLICATION NOTE Figure 2. Methylphenidate calibration curves for enantiomer R and S. Experimental Conditions Experiment
APCI (Nitrogen gas used for all connections)
animals dosed with racemic mixtures. Verapamil Methylphenidate Fluoxetine
the coronary vasodilator, verapamil. www.appliedbiosystems.com Figure 3. Fluoxetine calibration curves for enantiomer R and S. Figure 4. Verapamil, 0.1 ng/mL racemate concentration, extracted from Figure 5. Methylphenidate, 0.1 ng/mL racemate concentration, rat plasma. extracted from rat plasma. Results and Discussion
centration range of 0-1,000 ng/mL.
separation, approximately 65 minutes. www.appliedbiosystems.com Worldwide Sales Offices Applied Biosystems vast distribution
office locations, please call the division
at www.appliedbiosystems.com. Figure 6. Fluoxetine, 100 ng/mL racemate concentration, extracted from rat plasma and fluoxetine, 10 ng/mL racemate concentration, extracted from rat plasma plus integrating a switching valve to overcome mobile phase incom- Headquarters patibility. Note that the intensity is comparable despite the ten-fold decrease in analyte concentration. Conclusions
Not for use in diagnostic procedures.
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Printed in the USA, 12/2000, BHLPublication 114AP12-01
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British Journal of Haematology, 2002, 117, 768–774PROLONGED COMPLETE REMISSION IN TWO CASES OF ACUTE PROMYELOCYTIC LEUKAEMIATREATED WITH ATRA ALONEThe use of all-trans retinoic acid (ATRA) therapy forplatelet count was normal. On day 58, bone marrowinduction for acute promyelocytic leukaemia (APL) is wellshowed morphological remission, with normal cytogeneticsestablished and leads to a com
ß-Glucuronidase Immunotherapy in Dust Mite Allergic Children A Double-Blind Randomized Placebo- Controlled Trial With Short-Term ß-Glucuronidase Therapy in Children With Chronic Rhinoconjunctivitis and/or Asthma Due to Dust Mite E Galli,1 MS Bassi,2 E Mora,3 M Martelli,4 S Gianni,1 G Auricchio,1 1 Research Center San Pietro, Fatebenefratelli Hospital, AfaR, Rome, Italy4Santa
APPLICATION NOTE
Figure 2. Methylphenidate calibration curves for enantiomer R and S.
Figure 3. Fluoxetine calibration curves for enantiomer R and S.
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