Moltoxproductse2012

GENETIC TOXICOLOGY 2012
Quality Products for IN VITRO and GENETIC TOXICOLOGY
METABOLIC ACTIVATION PRODUCTS

CATALOG NO.
S9 SD rat liver Phenobarbital/Benzoflavone KCl frozen MutazymeTM 30% S9 Mix, rat liver Aroclor, lyoph. MutazymeTM 30% S9 Mix, rat liver Aroclor, lyoph. MutazymeTM 10% S9 Mix, rat liver Aroclor, lyoph. MutazymeTM 5% S9 Mix, rat liver Aroclor, lyoph. S9 GS hamster Aroclor KCl for modified Ames frozen S9 GS hamster Aroclor KCl for mod. Ames lyoph. S9 Mouse, please ask for availability!!
NADPH REGENERATING SYSTEM REAGENTS

CATALOG NO.

PREPARED BACTERIOLOGICAL MEDIA

CATALOG NO.
Miminimal Glucose Agar Plates 6 Well Plates
PREPARED TOP AGARS

CATALOG NO.

REAGENTS

CATALOG NO.

CONTROLCHEMTM PRODUCTS

CATALOG NO.


BACTERIAL STRAINS

CATALOG NO.
Strains are for use as culture inoculum. 71-097L ECDisc WP2 trp uvrA pKM101, lyoph. PTM™ Salmonella typhimurium TA1535psK1002**
Salmonella typhimurium strains for use in the bacterial mutagenesis assay described by Maron, D. and Ames., B., Mutation Research, 113:173-215, 1983. Escherichia coli strains (WP2 derivatives) for use in the bacterial mutagenesis assay described by Green, M.H.L. and Muriel, W.J., Mutation Research, 38:3-32, 1976. Please Note: The bacterial strains contained in STDiscs™ and ECDiscs™ are potential etiologic agents and are intended for use only by those skilled in the safe handling of potentially infectious agents. **• according to the German "Gentechnikgesetz" TA102 has been classified as "genetically diversified organism" (GVO) of risk group 1. Storage 2-8°C. BSL1. • Please check for appropriate regulations in your country. • For use as culture inocolum. Bacterial strains S. Typhimurium and E. coli are lyophilized and stabilized.

Ames Plate Incorporation Assay Kits

CATALOG NO.

31-100.2
Salmonella Mutagenicity Complete Test Kit
1 sample kit
STDisc™ TA97a, TA98, TA100 & TA102** (10 discs per strain/vial) Lyophilized S9, 2 vials/2,1 ml, Aroclor induced NADPH REGENSYS™ (Regensys A 50ml & Regensys B 50ml) ControlChem™ Mitomycin C, ICR191, Daunomycin, Sodium Azide & 2-Aminoanthracene Nutrient Broth, Histidine/Biotin Top Agar, ST Quad PC™ plates Minimal Glucose Agar Plates (160 dishes), Nutrient Agar plates (20 dishes) Application and Instruction Manual Includes the materials needed to perform the Ames Mutagenicity Plate Incorporation assay E. coli Mutagenicity Test Kit
1 sample kit
ECDisc™ WP2trp and WP2 trp uvrA (10 discs per strain/vial) Lyophilized S9 NADPH REGENSYS™ (Regensys A 15ml & Regensys B 15ml) ControlChem™ Methyl methanesulfonate & 2-Aminoanthracene Nutrient Broth, Tryptophan Top Agar, EC TriPC™ plates Minimal Glucose Agar Plates (80 dishes), Nutrient Agar plates (20 plates) Application and Instruction Manual Similar to the Salmonella Mutagenicity Complete Test Kit but with E. coli WP2 strains (trp, trp uvrA)
Microtiter Mutagenicity Fluctuation Assay Kits
31-300

Moltox FTTM TA98 & TA100 Mutagenicity Assay Kit
1 sample kit
STDisc™ TA98 & TA100 (2 discs per strain/vial) FT™ Growth Media, FT™ Exposure Media, FT™ Reversion Indicator Media MUTAZYME™ Moltox FT™ 30% S9 Mix, 3,25 ml, lyophilized rat liver, Aroclor induced Positive Controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide, 2-Nitrofluorene User Instructions Reagents provided are sufficient to perform 3 test materials with no replicates or 1 sample in triplicate, +/- S9 at various concentrations (≤6), positive controls and solvent (negative control). Moltox FTTM "471" Mutagenicity Assay Kit
1 sample kit
STDisc™ TA98, TA100, TA1535, TA1537, (2 x 2 discs/vial each) EC WP2 (trp, uvrA, pKM101), E. coli WP2 pKM101 (2 x 2 discs/vial each) FT™ Growth Media, FT™ Exposure Media, FT™ Reversion Indicator Media (2x 500 ml) Mutazyme™ 30% S9 Mix, 6.5 ml, lyophilized rat liver, Aroclor induced Positive Controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide, N4-aminocytidine, 2-Nitrofluorene, 9-Aminoacridine-hydrochloride Reagents provided are sufficient to analyze 1 sample in triplicate with all 6 strains in the presence and absence of S9 at various concentrations (≤6), positive controls and solvent (negative control).

CATALOG NO.
Moltox FTTM E. Coli Mutagenicity Assay Kit
1 sample kit
EC WP2 (pKM101) discs, EC WP2 (trp, uvrA, pKM101) (2 discs/vial each)
Ames FT™ Growth Media, FT™ Exposure Media, FT™ Reversion Indicator Media
Mutazyme™ 30% S9 Mix, 3,25 ml, lyophilized rat liver, Aroclor induced
Positive Controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide
Reagents provided are sufficient to test 1 sample in triplicate (or 3 samples with no replicate), +/- S9 at various concentrations (≤6), positive controls and solvent (negative control).

BioReliance Ames II™ Mutagenesis Assay

32-102
Ames II™ Mutagenicity Assay Kit by BioReliance™
1 sample kit
STDisc™ TA98 discs and TAMix discs (2 discs/vial each) Growth Media, Exposure Media, Indicator Media
Ampicillin, frozen
Mutazyme™ 30% S9 Mix AMES II™ Kits, lyophilized rat liver, Aroclor induced
Positive Controls: 2-Nitrofluorene, 4-Nitroquinoline oxide, 2-Aminoanthracene
Software disc and instruction manual
Reagents provided are sufficient to test 1 sample in triplicate (or 3 samples with no replicate), +/- S9 at various concentrations (≤6), positive controls and solvent (negative control). Plastic Ware for Moltox Ames FTTM Mutagenicity Assay Kit
or BioReliance Ames II Mutagenesis Assay
UMU Genotoxicity Test Kit

CATALOG NO.

Moltox UMU Genotoxicity Test Kit
1 sample kit
PTM™ Salmonella typhimurium TA1535pSK1002** (2 vials, 2 discs/vial each)
TGA Culture Medium, Ampicillin, B-buffer, Stop Reagent, ONPG, 2-Mercaptoethanol
Mutazyme™ 30% S9 Mix, 3,5 ml, lyophilized rat liver, Aroclor induced
Positive Controls: 2-Aminoanthracene, 4-Nitroquinoline-N-oxide
User Instruction
According ISO 13829 Reagents provided are sufficient to test 3 test materials with no replicates or 1 sample in triplicate, +/- S9 at various concentrations (≤6), positive controls and solvent (negative control). Consists of materials to perform the umu genotoxicity assay as described by Reifferscheid, G, et al., "A microplate version of the SOS/umu-test for rapid detection of genotoxins and genotoxic potentials of environmental samples", Mutation Research, 253:215-222, 1991.
Individual components for Mutagenicity Assays

CATALOG NO.

30% Mutazyme™ S9 Mix for Moltox FT™ Kits 30% Mutazyme™ S9 Mix for Moltox FT™ Kits PTM™ Salmonella typhimurium TA1535psK1002**
**• according to the German "Gentechnikgesetz" TA1535psK has been classified as "genetically diversified organism" (GVO) of risk group 1. Please check for appropriate regulations in your country. Salmonella strain for use in the SOS/umu-test described by Oda, Y., S. Nakamura, J. Oki and T. Kato, Mutation Research, 147:219-119, 1985. Offered in proprietary transport medium (PTM™).
AMES II TM INDIVIDUAL COMPONENTS
AMES II TM is a Brand of BioReliance. Components are produced by Moltox.
CATALOG NO.
30% Mutazyme™ S9 Mix for AMES IITM Kits
Please ask for customized high throughput screening materials!
Bacterial strains S. Typhimurium are lyophilized and stabilized. For use as culture inoculum. Storage 2-8°C.
More about Moltox
The above list is representative of the products we have on stock in Europe. For more MolTox products please visit
www.moltox.com. The products are used by researchers and industrial microbiologists in the fields of Genetic and in vitro toxicology
and additional metabolic studies. Moltox welcomes the opportunity to provide you with custom formulations of S9, prepared
bacteriological media and top agars. Preparation of these items in house can be costly and time consuming. Our labs provide a lower
cost, on time solution.
In addition, each of our products are accompanied by GLP-compliant Production & Formulation and Quality Assurance Certificates.
Your assurance of Quality and Performance.

Shipping
All international dry ice and blue ice shipments will be made Monday through Wednesday. Domestic dry ice and blue ice shipments
will be made Monday through Wednesday within 1-2 days. All other items may be shipped Monday through Thursday.
Transportation of frozen shipments (Frozen S9) within Europe are to be done by TNT unless otherwise requested. Packing charges
(incl. dry ice and the fees for the dangerous good dry ice) apply to dry and blue ice shipments.
Cost of cold shipments (lyophilized S9, NADPH, straines, media, controls, etc) are dependant on weight and volume.
Please contact our office for details. Room temperature shipments may be shipped within 2-4 days.

Source: http://www.trinova.de/Gentoxikologie/MoltoxProducts2012.pdf

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