Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
EFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MEN
Amin Mohammadi Domieh1 and Ali Khajehlandi2
1 Department of Exercise physiology, Islamic Azad University, Gachsaran branch, Iran.
2 Department of Exercise physiology, Islamic Azad University, Gachsaran branch, Iran. Corresponding author: Amin Mohammadi Domieh (MS) Department of Exercise Physiology, Islamic Azad University, Gachsaran branch, Iran Tel: +98- 07422229358 Fax: +98-07423330317 PO BOX: 75817-75785 E-mail: Aminmohammadi@iaug.ac.ir Submitted for publication: Jul 2010 Accepted for publication: Aug 2010 ABSTRACT DOMIEH, A. M.; KHAJEHLANDI, A. Effect of 8 weeks endurance training on plasma visfatin in middle-aged men Brazilian Journal of Biomotricity, v. 4, n. 3, p. 174-179, 2010. Visfatin, a novel adipokine, was increased in obesity. It is not well known whether endurance training - induced change in adipose tissue and blood lipids decreases plasma visfatin or not; thus, the purpose of this study was to examine the effect of 8 weeks endurance training on plasma visfatin in middle-aged men. Nineteen healthy middle-aged men (aged 37.5 ± 4.8 years; height 173.7 ± 6.5 cm; BMI 25.2 ± 2.6 kg/m2; mean ± SD) participated as subjects in this study. The subjects were randomly assigned to endurance training group (n=9) or control group (n=10). Endurance training group underwent an 8-week intervention, with a frequency of 3 d/wk at an intensity corresponding to 65 - 80% maximum heart rate for 20 - 34 min. The results showed that body fat percent, WHR and plasma visfatin were decreased (P<0.05) after endurance training. Maximum oxygen consumption, on the other hand, increases significant (P<0.05) in the training group compared with the control group. Plasma visfatin levels at baseline were positively correlated (P<0.05) with body fat percent and triglyceride concentration. In conclusion, it seems that 8 weeks endurance training induced change in adipose tissue, decrease plasma , v. 4, n. 3, p. 174-179, 2010 (ISSN 1981-6324)
visfatin in middle-aged men. Key words: Endurance training, Plasma visfatin, Dislipidemia, Middle-aged men. otricity m INTRODUCTION
Visfatin is a recently discovered adipokine, which is highly expressed in visceral fat. Adipocyte visfatin expression and plasma concentrations increase with obesity in animals (FUKUHARA et al., 2005) and humans (BERNDT et al., 2005). The metabolic effects of
urnal of Bio
visfatin are apparently mediated by the binding to and activation of the insulin receptor (FUKUHARA et al., 2005). Visfatin exerts insulin-mimetic effects and some studies suggest that visfatin is related to the development of the metabolic syndrome (CHEN et al.,
Brazilian Jo
Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
2006). Interestingly, visfatin treatment did not promote insulin resistance, but exhibited an insulin mimetic effect. Visfatin treatment in diabetic mice improved insulin sensitivity in vivo and resulted in decreased glucose and insulin levels (FUKUHARA et al., 2005). Studies demonstrated that pioglitazone reduces the visfatin expression (LV et al., 2009), but visfatin unaffected from rosiglitazone and metformin (KADOGLOU et al., 2010). Several studies exploring the effects of exercise on visfatin mRNA and plasma concentrations have results inconsistent findings. For example, Frydelund-Larsen et al demonstrated that visfatin mRNA expression increases after an exhaustion exercise and remained elevated 24 h after exercise. Plasma visfatin, on the other hand, not affected (BO et al., 2009) or decreased (HAIDER et al., 2006; HAUS et al., 2009) in response to exercise training. It is not well known whether exercise - induced change in adipose tissue - decreases plasma visfatin; therefore, we examined the effects of 8 weeks endurance training on plasma visfatin in middle-aged men.
MATERIALS AND METHODS
Nineteen healthy middle-aged men (mean ± SD: 37.5 ± 4.8 years), volunteered to participate in this study. Written informed consent was signed by all participants. The Islamic Azad University of Gachsaran Ethics Committee approved the protocols, which were fully explained to all subjects. The subjects were randomly assigned to a control group (n=10) or training group (n=9).
The subjects in the training group underwent an 8-week intervention, with a frequency of 3 d/wk at an intensity corresponding to 65-80% maximum heart rate for 20-34 min.
Anthropometric and body composition measurements
Height and body weight were measured, and body mass index (BMI; kg/m2) was calculated from height and weight of each subject. Waist circumference was determined by obtaining the minimum circumference (narrowest part of the torso, above the umbilicus) and the maximum hip circumference while standing with their heels together. The waist to hip ratio (WHR) was calculated by dividing waist by hip circumference (cm) (ACSM, 2005). Subcutaneous body fat was measured at 3 sites (chest, abdominal, and thigh) with a Lafayette caliper. Body fat percent was calculated from the formula developed by Jackson and Pollock (JACKSON et al, 1980). All subjects fasted at least for 12 hours and a fasting blood sample was obtained by venipuncture. The plasma visfatin level was measured in
, v. 4, n. 3, p. 174-179, 2010 (ISSN 1981-6324)
duplicate using an enzyme-linked immunosorbent assay (ELISA) kits (Uscn Life Science Inc, Wuhan, China). Serum cholesterol triglycerides, HDL-c and LDL-c were assayed with automated techniques.
otricity m
Statistical analyses were performed with SPSS program (version 13, SPSS, Inc., Chicago, IL). Values were expressed as mean ± standard deviation (SD). Independent t-test and paired t-test were used to evaluate changes in variables. General linear regression analysis and Pearson's correlation were performed to calculate a correlation between
urnal of Bio
variables in response to training. P-values less than 0.05 were considered statistically significant.
Brazilian Jo
Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
Anthropometric, physiological and metabolic characteristics of subjects are shown in Table 1. The results showed that body weight, body mass index, body fat percent and WHR were decreased (P<0.05) after endurance training. Maximum oxygen consumption, on the other hand, increases significant (P<0.05) in the training group compared with the control group. Plasma visfatin and LDL-c decreased (P<0.05) and HDL-c increased (P<0.05) after 8 weeks endurance training (Table 1). Pearson's correlation demonstrated a positive relationship between plasma visfatin levels at baseline (P<0.05) with body fat percent and triglyceride concentration.
Table 1 - Anthropometric and metabolic characteristics of study subjects (mean ± SD) Training Variables Post test Post test
* P<0.05 for between-group differences; † P<0.05, pretraining vs. posttraining values.
DISCUSSION
The results showed that Plasma visfatin decreased (P<0.05, 29.6%) in response to 8 weeks endurance training compared to the control group. Recently, Fukuhara et al. identified visfatin as a new adipokine that is preferentially produced in the visceral adipose tissue of obese mice and humans. Although the function of visfatin is not currently understood, visfatin may have a dual role: an autocrine/paracrine function that facilitates differentiation and fat deposition on visceral adipose tissue, and an endocrine role that modulate insulin sensitivity in peripheral organs (SETHI et al., 2005). Therefore, visfatin may facilitate glucose control; on the other hand, it may promote the development of obesity (ARNER, 2006). Researchers suggested that improving body composition and adipose tissue may effective mechanisms for decrease circulating levels of visfatin (HAUS et al., 2009). Waist-hip circumferences and abdominal adipose tissue are independent
, v. 4, n. 3, p. 174-179, 2010 (ISSN 1981-6324)
predictors for plasma visfatin (BO et al., 2009; SUN et al., 2005). Endurance exercise - induced change in visceral adipose tissue, increases plasma adiponectin (FU et al., 2005)
otricity
and leptin (RYAN et al., 2000). The results showed that body fat percent and WHR
m
decreased after endurance training, thus exercise-induced changes in body fat, especially visceral adipose tissue, may attribute to plasma visfatin decrease. On the other hand, there was the positive relationship between plasma visfatin and body fat percent at baseline and after the training. In another study of Berndt et al found a positive correlation
urnal of Bio
between plasma visfatin concentration and %BF measured by DXA. It is encouraging that only a modest loss of 5-10 percent of body fat percent, leading to simultaneous improvement in adipokines (FREEDLAND, 2004). Exercise without weight loss is effective
Brazilian Jo
Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
in reducing visceral adipose tissue and preventing further increases in obesity (ROSS et al., 2000). Body fat percent decreased 8.8% after 8 weeks endurance training, thus it seems that the endurance training could offer a sufficient stimulus for plasma visfatin decreases. Although some studies suggest that visfatin is related to the development of the metabolic syndrome (FUKUHARA et al., 2005), others reported that visfatin gene expression was not associated with the metabolic syndrome in diseased rats when compared with lean controls (KLOTING et al., 2005). In the present study, Pearson's correlation demonstrated a positive relationship between plasma visfatin levels at baseline with triglyceride concentration. Sun et al demonstrated that triglyceride was the only significant predictor of baseline visfatin concentrations independent of age and body fat percent. Our data in confirm to another investigations suggest that visfatin may exert an independent role in regulating triglyceride metabolism in humans (SUN et al., 2005). Jian et al reported that a single nucleotide polymorphism at different loci of visfatin gene was associated with triglyceride and total cholesterol levels. These reports suggest that visfatin may play a role in lipid homeostasis; however, the underlying mechanism is currently unknown (JIAN et al., 2006). Generally, plasma visfatin may relate to the development of the metabolic syndrome. We have some limitation in this study. Visfatin exerts insulin-mimetic effects and some studies suggested that plasma visfatin may related to fasting glucose and insulin and inflammatory markers such as TNF-α and IL-6 (). Therefore, additional researches are needed to determine the effects of exercise training on plasma visfatin, type 2 diabetes risk factors, inflammatory markers and their relationships.
CONCLUSIONS AND PRACTICAL APPLICATION
In conclusion, endurance training induced change in adipose tissue, decrease plasma visfatin in middle-aged men. These findings suggested that changes in visfatin levels may be associated with the beneficial effect of exercise. Further studies are needed to elucidate the mechanisms responsible for the effects of exercise on visfatin.
REFERENCES
ACSM. Guidelines for exercise testing and prescription. Philadelphia: Lippincott Williams & Wilkins. 57-90, 2005. ARNER, P. Visfatin-a true or false trail to type 2 diabetes mellitus. Journal of Clinical Endocrinology and Metabolism, v. 91, p. 28-30, 2006. BERNDT, J.; KLOTING, N., KRALISCH, S., KOVACS, P., FASSHAUER M., SCHON, M.
, v. 4, n. 3, p. 174-179, 2010 (ISSN 1981-6324)
R., STUMVOLL, M. BLUHER, M. Plasma visfatin concentrations and fat depot-specific mRNA expression in humans. Diabetes, v. 54, p. 2911-2916, 2005.
otricity
BO, S.; CICCONE, G.; BALDI, I.; GAMBINO, R.; MANDRILE, C.; DURAZZO, M.;
m
GENTILE, L.; CASSADER, M.; CAVALLO-PERIN, P.; PAGANO, G. Plasma visfatin concentrations after a lifestyle intervention were directly associated with inflammatory markers. Nutrition, Metabolism and Cardiovascular Disease, v. 19, p. 423-30, 2009. CHEN, M. P.; CHUNG, F. M.; CHANG, D. M.; TSAI, J. C. R.; HUANG, H. R.; SHIN, S. J.;
urnal of Bio
LEE, Y. J. Elevated Plasma Level of Visfatin/Pre-B Cell Colony- Enhancing Factor in Patients with Type 2 Diabetes Mellitus. Journal of Clinical Endocrinology and Metabolism, v. 91, p. 295-299, 2006.
Brazilian Jo
Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
CHOI, K. M.; KIM, J. H.; CHO, G. J.; BAIK, S. H.; PARK, H. S.; KIM, S. M. Effect of exercise training on plasma visfatin and eotaxin levels. European Journal of Endocrinology, v. 157, p. 437-442, 2007. DAVUTOGLUA, M.; OZKAYAB, M.; GULERA, E.; GARIPARDICA, M.; GURSOYA, H.; KARABIBERA, H.; KILINC, M. Plasma visfatin concentrations in childhood obesity: relationships to insulin resistance and anthropometric indices. Swiss Medical Weekly, v. 139, p. 22-27, 2009. FREEDLAND, E. S. Role of a critical visceral adipose tissue threshold (CVATT) in metabolic syndrome: implications for controlling dietary carbohydrates: a review. Nutrition & Metabolism, v. 1, p. 1-24, 2004. FRYDELUND-LARSEN, L.; AKERSTROM, T.; NIELSEN, S.; KELLER, P.; KELLER, C.; PEDERSEN, B. K. Visfatin mRNA expression in human subcutaneous adipose tissue is regulated by exercise. American Journal of Physiology Endocrinology Metabolism, v. 292, p. E24-E31, 2007. FU, Y.; LUO, N.; KLEIN, R.; GARVEY, W. T. Adiponectin promotes adipocyte differentiation, insulin sensitivity, and lipid accumulation. Journal of Lipid Research, v. 46, p. 1369-1379, 2005. FUKUHARA, A.; MATSUDA, M.; NISHIZAWA, M.; SEGAWA, K.; TANAKA, M.; KISHIMOTO, K.; MATSUKI, Y. Visfatin: a protein secreted by visceral fat that mimics the effects of insulin. Science, v. 307, p. 426-430, 2005. HAIDER, D. G.; PLEINER, J.; FRANCESCONI, M.; WIESINGER, G. F.; MÜLLER, M.; WOLZT, M. J. Exercise training lowers plasma visfatin concentrations in patients with type 1 diabetes. Journal of Clinical Endocrinology and Metabolism, v. 91, p. 4702-4704, 2006. HAUS, J. M.; SOLOMON, T. P.; MARCHETTI, C. M.; O'LEARY, V. B.; BROOKS, L. M.; GONZALEZ, F.; KIRWAN, J. P. Decreased visfatin after exercise training correlates with improved glucose tolerance. Medicine and Science in Sports and Exercise, v. 41, p. 1255-1260, 2009. JACKSON, A. S.; POLLOCK, M. L.; WARD, A. Practical assessment of body composition. Physician Sportsmed, v. 13, p. 76-90, 1980. JIAN, W. X.; LUO, T. H.; GU, Y. Y. The visfatin gene is associated with glucose and lipid metabolism in a Chinese population. Diabetes Medicine, v. 23, p. 967-73, 2006. KADOGLOU, N. P.; TSANIKIDIS, H.; KAPELOUZOU, A.; VRABAS, I.; VITTA, I.; KARAYANNACOS, P. E.; LIAPIS, C. D., SAILER, N. Effects of rosiglitazone and metformin treatment on apelin, visfatin, and ghrelin levels in patients with type 2 diabetes mellitus. Metabolism, v. 59, p. 373-379, 2010. KLOTING, N.; KLOTING, I. Visfatin: gene expression in isolated adipocytes and sequence
, v. 4, n. 3, p. 174-179, 2010 (ISSN 1981-6324)
analysis in obese WOKW rats compared with lean control rats. Biochemica et Biophysica Acta, v. 332, p. 1070-1072, 2005.
otricity
LV, Q.; WANG, Y.; WANG, W.; WANG, L.; ZHOU, X. Effect of pioglitazone on visfatin
m
expression in 3T3-L1 adipocytes and SD rats. Endocrinology Research, v. 34, p. 130-141, 2009. NEIMAN, D. C. Fitness and sports medicine: An introduction. Bull Publishing Company,
urnal of Bio
1990. NICKLAS, B. J.; BERMAN, D. M. Endurance exercise and adipose tissue. CRC Press LLC, 2002.
Brazilian Jo
Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
ROSS, R.; DAGNONE, D.; JONES, P. G.; SMITH, H.; PADDAGS, A.; HUDSON, R.; JANSSEN, I. Reduction in obesity and related comorbid conditions after diet-induced weight loss or exercise-induced weight loss in men: A randomized, controlled trial. Annals of Internal Medicine, v. 133, p. 92-103, 2000. RYAN, A. S.; PRATLEY, R. E.; ELAHI, D.; GOLDBERG, A. P. Changes in plasma leptin and insulin action with resistive training in postmenopausal women. International Journal of Obesity and Related Metabolic Disorders, v. 24, p. 27-32, 2000. SEO, D.; YOO, E. J.; LIM, K.; SUK. M. H.; JUN, T. W.; SONG, W. Plasma visfatin response to combined exercise training in healthy women. FASEB Journal, v. 21, p. 765, 2007. SETHI, J.; VIDAL-PUIG, A. Visfatin: the missing link between intraabdominal obesity and diabetes? Trends in Molecular Medicine, v. 11, p. 344-347, 2005. SUN, G.; BISHOP, J.; KHALILI, S.; VASDEV, S.; GILL, V.; PACE, D.; FITZPATRICK, D.; RANDELL, E.; XIE, Y. G.; ZHANG, H. Serum visfatin concentrations are positively correlated with serum triacylglycerols and down-regulated by overfeeding in healthy young men. American Journal of Clinical Nutrition, v. 85, p. 399-404, 2005.
, v. 4, n. 3, p. 174-179, 2010 (ISSN 1981-6324) otricity m urnal of Bio Brazilian Jo
MATERIAL SAFETY DATA SHEET AMPICILLIN, SODIUM SALT I IDENTIFICATION: V FIRE AND EXPLOSION HAZARDS: BioShop Canada Inc. Flammable : No 4060 Fairview St Unit #6-8 Flash Point : N/A Burlington, OntarioL7L 4Y8 Extinguishing Media: Water, dry chemical, chemical foam or alcohol 1-800-234-1179 resistant foam. Explosion Data : Not available Emergency Telephone No.: 905-4
Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
EFFECT OF 8 WEEKS ENDURANCE 
Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
2006). Interestingly, visfatin treatment did not promote insulin resistance, but exhibited an insulin mimetic effect. Visfatin treatment in diabetic mice improved insulin sensitivity in vivo and resulted in decreased glucose and insulin levels (FUKUHARA et al., 2005). Studies demonstrated that pioglitazone reduces the visfatin expression (LV et al., 2009), but visfatin unaffected from rosiglitazone and metformin (KADOGLOU et al., 2010). Several studies exploring the effects of exercise on visfatin mRNA and plasma concentrations have results inconsistent findings. For example, Frydelund-Larsen et al demonstrated that visfatin mRNA expression increases after an exhaustion exercise and remained elevated 24 h after exercise. Plasma visfatin, on the other hand, not affected (BO et al., 2009) or decreased (HAIDER et al., 2006; HAUS et al., 2009) in response to exercise training. It is not well known whether exercise - induced change in adipose tissue - decreases plasma visfatin; therefore, we examined the effects of 8 weeks endurance training on plasma visfatin in middle-aged men.
MATERIALS AND METHODS 
Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
Anthropometric, physiological and metabolic characteristics of subjects are shown in Table 1. The results showed that body weight, body mass index, body fat percent and WHR were decreased (P<0.05) after endurance training. Maximum oxygen consumption, on the other hand, increases significant (P<0.05) in the training group compared with the control group. Plasma visfatin and LDL-c decreased (P<0.05) and HDL-c increased (P<0.05) after 8 weeks endurance training (Table 1). Pearson's correlation demonstrated a positive relationship between plasma visfatin levels at baseline (P<0.05) with body fat percent and triglyceride concentration.
Table 1 - Anthropometric and metabolic characteristics of study subjects (mean ± SD) 
Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
in reducing visceral adipose tissue and preventing further increases in obesity (ROSS et al., 2000). Body fat percent decreased 8.8% after 8 weeks endurance training, thus it seems that the endurance training could offer a sufficient stimulus for plasma visfatin decreases. Although some studies suggest that visfatin is related to the development of the metabolic syndrome (FUKUHARA et al., 2005), others reported that visfatin gene expression was not associated with the metabolic syndrome in diseased rats when compared with lean controls (KLOTING et al., 2005). In the present study, Pearson's correlation demonstrated a positive relationship between plasma visfatin levels at baseline with triglyceride concentration. Sun et al demonstrated that triglyceride was the only significant predictor of baseline visfatin concentrations independent of age and body fat percent. Our data in confirm to another investigations suggest that visfatin may exert an independent role in regulating triglyceride metabolism in humans (SUN et al., 2005). Jian et al reported that a single nucleotide polymorphism at different loci of visfatin gene was associated with triglyceride and total cholesterol levels. These reports suggest that visfatin may play a role in lipid homeostasis; however, the underlying mechanism is currently unknown (JIAN et al., 2006). Generally, plasma visfatin may relate to the development of the metabolic syndrome. We have some limitation in this study. Visfatin exerts insulin-mimetic effects and some studies suggested that plasma visfatin may related to fasting glucose and insulin and inflammatory markers such as TNF-α and IL-6 (). Therefore, additional researches are needed to determine the effects of exercise training on plasma visfatin, type 2 diabetes risk factors, inflammatory markers and their relationships.
CONCLUSIONS AND PRACTICAL APPLICATION 
Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
CHOI, K. M.; KIM, J. H.; CHO, G. J.; BAIK, S. H.; PARK, H. S.; KIM, S. M. Effect of exercise training on plasma visfatin and eotaxin levels. European Journal of Endocrinology, v. 157, p. 437-442, 2007. DAVUTOGLUA, M.; OZKAYAB, M.; GULERA, E.; GARIPARDICA, M.; GURSOYA, H.; KARABIBERA, H.; KILINC, M. Plasma visfatin concentrations in childhood obesity: relationships to insulin resistance and anthropometric indices. Swiss Medical Weekly, v. 139, p. 22-27, 2009. FREEDLAND, E. S. Role of a critical visceral adipose tissue threshold (CVATT) in metabolic syndrome: implications for controlling dietary carbohydrates: a review. Nutrition & Metabolism, v. 1, p. 1-24, 2004. FRYDELUND-LARSEN, L.; AKERSTROM, T.; NIELSEN, S.; KELLER, P.; KELLER, C.; PEDERSEN, B. K. Visfatin mRNA expression in human subcutaneous adipose tissue is regulated by exercise. American Journal of Physiology Endocrinology Metabolism, v. 292, p. E24-E31, 2007. FU, Y.; LUO, N.; KLEIN, R.; GARVEY, W. T. Adiponectin promotes adipocyte differentiation, insulin sensitivity, and lipid accumulation. Journal of Lipid Research, v. 46, p. 1369-1379, 2005. FUKUHARA, A.; MATSUDA, M.; NISHIZAWA, M.; SEGAWA, K.; TANAKA, M.; KISHIMOTO, K.; MATSUKI, Y. Visfatin: a protein secreted by visceral fat that mimics the effects of insulin. Science, v. 307, p. 426-430, 2005. HAIDER, D. G.; PLEINER, J.; FRANCESCONI, M.; WIESINGER, G. F.; MÜLLER, M.; WOLZT, M. J. Exercise training lowers plasma visfatin concentrations in patients with type 1 diabetes. Journal of Clinical Endocrinology and Metabolism, v. 91, p. 4702-4704, 2006. HAUS, J. M.; SOLOMON, T. P.; MARCHETTI, C. M.; O'LEARY, V. B.; BROOKS, L. M.; GONZALEZ, F.; KIRWAN, J. P. Decreased visfatin after exercise training correlates with improved glucose tolerance. Medicine and Science in Sports and Exercise, v. 41, p. 1255-1260, 2009. JACKSON, A. S.; POLLOCK, M. L.; WARD, A. Practical assessment of body composition. Physician Sportsmed, v. 13, p. 76-90, 1980. JIAN, W. X.; LUO, T. H.; GU, Y. Y. The visfatin gene is associated with glucose and lipid metabolism in a Chinese population. Diabetes Medicine, v. 23, p. 967-73, 2006. KADOGLOU, N. P.; TSANIKIDIS, H.; KAPELOUZOU, A.; VRABAS, I.; VITTA, I.; KARAYANNACOS, P. E.; LIAPIS, C. D., SAILER, N. Effects of rosiglitazone and metformin treatment on apelin, visfatin, and ghrelin levels in patients with type 2 diabetes mellitus. Metabolism, v. 59, p. 373-379, 2010. KLOTING, N.; KLOTING, I. Visfatin: gene expression in isolated adipocytes and sequence
, v. 4, n. 3, p. 174-179, 2010 (ISSN 1981-6324) 
Domieh et al.: Endurance training and plasma visfatin www.brjb.com.br
ROSS, R.; DAGNONE, D.; JONES, P. G.; SMITH, H.; PADDAGS, A.; HUDSON, R.; JANSSEN, I. Reduction in obesity and related comorbid conditions after diet-induced weight loss or exercise-induced weight loss in men: A randomized, controlled trial. Annals of Internal Medicine, v. 133, p. 92-103, 2000. RYAN, A. S.; PRATLEY, R. E.; ELAHI, D.; GOLDBERG, A. P. Changes in plasma leptin and insulin action with resistive training in postmenopausal women. International Journal of Obesity and Related Metabolic Disorders, v. 24, p. 27-32, 2000. SEO, D.; YOO, E. J.; LIM, K.; SUK. M. H.; JUN, T. W.; SONG, W. Plasma visfatin response to combined exercise training in healthy women. FASEB Journal, v. 21, p. 765, 2007. SETHI, J.; VIDAL-PUIG, A. Visfatin: the missing link between intraabdominal obesity and diabetes? Trends in Molecular Medicine, v. 11, p. 344-347, 2005. SUN, G.; BISHOP, J.; KHALILI, S.; VASDEV, S.; GILL, V.; PACE, D.; FITZPATRICK, D.; RANDELL, E.; XIE, Y. G.; ZHANG, H. Serum visfatin concentrations are positively correlated with serum triacylglycerols and down-regulated by overfeeding in healthy young men. American Journal of Clinical Nutrition, v. 85, p. 399-404, 2005.
, v. 4, n. 3, p. 174-179, 2010 (ISSN 1981-6324)